Figure 3. Quantitative Real Time PCR Determination of Pallidin Transcripts in Neurons derived from Human IPSCs.

(A) Experimental design to generate monoallellic neurons from MECP2 mutant Rett syndrome patient cells. (B) Wild type and mutant monoallellic IPSCs from the same patient were differentiated into neurons and analyzed by qRT-PCR. Plot depicts ratio of mRNA content between the wild type and mutant monoallellic cells from the same subject (n = 2 patients). P values were obtained by One Way Anova with Dunnett’s Multiple comparisons correction.