Figure 2.(A and B) Wt-MiaPaCa2 (A), si-MiaPaCa2 (A), BxPC-3 (B) and Panc-1 (B) pancreatic cancer cells were incubated with different amounts of glucose in hypoxia or normoxia for six hours. HIF-1α was determined by western blotting, using GAPDH, Topo1 and β-actin as loading controls. When necessary, hypoxic wt-MiaPaCa2 cells were used as a positive control (PC). (C) Wt-MiaPaCa2 cells were incubated in hypoxia for 6 h, using media with 5.6-, 16.7- and 22.2 mM glucose. Then, the cells were incubated in normoxia for 0-, 5- or 10 min using the same media. Afterwards, HIF-1α was determined by western blotting.