Figure 4. AK3 and AK10 induce a G₂/M arrest on their own and sub-diploid cell fragments in the presence of TNF. HT29 cells treated with AK3 or AK10 in the presence or absence of TNF were analyzed for DNA content by propidium iodide staining. Flow cytometric analysis of AK3 and AK10 treated cells showed a shift from G₁ to G₂/M. In the presence of TNF alone, little change in the flow cytometer pattern is observed. Combining TNF with AK3 and AK10 showed a decrease in the G₂/M peak and the appearance of a sub-diploid population.