Figure 3. Protective effects of GTM-1 in neuroblastoma cells.

A–C, MC65 cells were grown in the presence (Tet+) or absence (Tet−) of tetracycline and under Tet− with GTM-1 for 24 hrs, and assessed for: (A) H₂O₂ production, (B) superoxide production. C, The effect of GTM-1 on the insoluble or soluble Aβ oligomers level were assessed using ELISA in MC65 cells 72 hrs after tetracyline withdrawal (C). Cells incubated with tetracycline (Tet+) were used as a negative control (NC), and cells cultured without tetracycline (Tet−) were used as a positive control (PC). D, SH-SY5Y cells were incubated with the indicated compounds for 24 h. The cell viability was assayed using the MTT assay. Aβ, Aβ42 (30 µM), 3-MA (10 µM), spautin-1 (10 µM), GTM-1 (20 µM). Cells treated with vehicle (0.1% DMSO) were used as a negative control (NC). E, MC65 cells were grown in the presence (Tet+) or absence (Tet−) of tetracycline and under Tet− with the indicated compounds for 24 hrs, and the cell viability was assessed using the MTT assay. 3-MA (10 µM), spautin-1 (10 µM), GTM-1 (20 µM). Cells cultured in the presence of tetracycline (Tet+) were used as a negative control (NC). Stars indicated significant differences between the model group and control or treatment groups for specific time points. *P<0.05, **P<0.01.