Table 1. Cell death induction upon (co-)expression of various RLK-encoding genes in Nicotiana leaves.
Construct | Cell death induction | ||
Separate expression | Co-expression with MtNFP-FP # | Co-expression with MtLYK3-FP # | |
MtNFP–sYFP2 | 0/12 | Not applicable | 20/22 |
MtNFP –3xFLAG | 0/9 | Not applicable | 12/13* |
MtNFP | 0/9 | Not applicable | 8/9** |
MtLYK3–sYFP2 | 0/12 | 20/22 | Not applicable |
MtLYK3–3xFLAG | 0/9 | 12/13* | Not applicable |
MtLYK3 | 0/9 | 8/9** | Not applicable |
MtDMI2-sYFP2 | 0/12 | 0/12 | 0/12 |
MtLRRII.1-YFP C | 0/9 | 0/9*** | 0/9*** |
AtBRI1- YFP C | 0/7 | 0/9*** | 0/9*** |
# − unless stated differently: with -3xFLAG (*) untagged (**), or −YFP N (***) tagged construct.
Indicated constructs were expressed alone or co-expressed with either MtNFP or MtLYK3 in Nicotiana leaves, and the infiltrated regions were marked. Macroscopic symptoms of cell death were scored 48 hai: only infiltrations that resulted in confluent death of (nearly) the entire infiltrated region were scored and are presented as a fraction of total infiltrations performed.