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. 2013 Jun 4;11(6):e1001572. doi: 10.1371/journal.pbio.1001572

Figure 4. Hiw-Wnd pathway regulates the expression and transcriptional activity of the C4da-specific transcription factor Kn.

Figure 4

(A) Overexpressing Wnd attenuates the nuclear Kn expression levels. Representative immunofluorescence images of wt and Wnd-overexpressing (OE Wnd) ddaC neurons labeled with antibodies against Kn (top) and Elav (bottom). Scale bar, 5 µm. (B) Quantification of the immunofluorescence intensity of nuclear Kn normalized to that of nuclear Elav in wt, hiwΔN, and OE Wnd neurons. (C) Wnd overexpression down-regulates the promoter activity of the ENaC ion channel pickpocket (ppk), a known target of Kn. Representative ddaC neurons labeled with ppk-eGFP in neurons of the following genotypes: (1) wt; (2) hiwΔN; (3) OE Wnd; (4) OE Kn; (5) hiwΔN+OE Kn; (6) OE Kn+OE Wnd. Scale bar, 5 µm. (D) Quantification of ppk-eGFP fluorescent intensity in neurons of the following genotypes: (1) wt; (2) hiwΔN; (3) OE Wnd; (4) knKN1/KN4; (5) OE Kn; (6) hiwΔN+OE Kn; (7) OE mCD8RFP+OE Kn; (8) OE Kn+OE Wnd.