Figure 5. Kn specifically mediates Hiw regulation of dendritic growth.

(A) hiw and kn interact genetically. Shown are representative dendrites of the following genotypes: (1) hiw^ΔN heterozygote (hiw^ΔN/+); (2) kn ^KN4 heterozygote (kn^KN4/+); (3) hiw^ΔN and kn^KN4 trans-heterozygote (hiw^ΔN/+; kn^KN4/+). Scale bar, 50 µm. (B) Quantification of total dendrite length of denoted genotypes. wt samples used for statistical analysis are the same as those in Figure 1. (C and C′) Overexpressing Kn partially rescues dendritic defects in hiw^ΔN mutants. Representative dendrites (C) and tracings (C′) of ddaC MARCM clones of following genotypes: (1) wt; (2) hiw^ΔN; (3) overexpressing Kn with MARCM (OE Kn); (4) overexpressing Knot in hiw^ΔN genetic background with MARCM (hiw^ΔN+OE Kn). Scale bar, 50 µm. (D) Quantification of total dendrite length (left) and number of dendrite termini (right). (E) Overexpressing Kn does not alter axon terminal morphology in hiw^ΔN mutants. Shown are representative axon terminals of ddaC MARCM clones of the indicated genotypes. Scale bar, 10 µm. (F) Quantification of the length of axon terminals.