Figure 6. Wnd kinase inhibits dendrite growth in C1da neurons expressing ectopic Kn.

(A) Wnd overexpression does not alter dendrite morphology in wild-type C1da neurons, but restrains the dendritic overgrowth caused by ectopic Kn in these neurons. Shown are representative dendrites of C1da neurons ddaD (left) and ddaE (right), labeled by Gal4^2-21/UAS-mCD8::GFP, of the following genotypes: (1) wt; (2) overexpressing Kn by Gal4^2-21(OE Kn); (3) hiw^ΔN homozygotes (hiw); (4) overexpressing Wnd by Gal4^2-21 (OE Wnd); (5) overexpressing Kn and Wnd by Gal4^2-21 (OE Kn+Wnd); (6) overexpressing Kn and a kinase-dead form of Wnd by Gal4^2-21(OE Kn+Wnd^KD). Scale bar, 50 ⋯µm. Magnified views of the boxed areas are shown on the right for each genotype. (B) Quantification of total dendrite length (left) and number of dendrite termini (right) of ddaEs of denoted genotypes. (C) Wnd kinase specifically down-regulates the expression of UAS-Kn, but not UAS-RedStinger (a nuclear red fluorescent protein) [66] in a posttranscriptional manner. Representative images of ddaEs labeled with antibodies against Kn (top) and RedStinger (bottom) in “OE Kn+Wnd” and “OE Kn+Wnd^KD” using Gal4^2-21. Scale bar, 5 µm. (D) Quantification of immunofluorescence intensity of nuclear Kn normalized to that of RedStinger. Two different Gal4 lines, Gal4^2-21 (left) and Gal4^21-7 (right), were tested in this experiment.