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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: Sci Transl Med. 2012 Feb 15;4(121):121ra18. doi: 10.1126/scitranslmed.3003205

Fig. 3.

Fig. 3

Gene targeted mutation indicates that microRNA 21 amplifies injury and fibrosis in the kidney. (A) Panels showing low power images of d10 UUO injured or sham treated kidneys from miR-21-/- or miR-21+/+ matched mice. (B) Quantification of Sirius red stained fibrosis in kidneys. (C) Q-PCR quantification of Coll1a1 and Coll3a1 transcripts normalized to Gapdh. (D) Number of apoptotic cells per high power field on TUNEL stained kidney sections. (E) Epithelia injury scores; either number of epithelial cells with brush border or number of tubules with cells containing brush border. (F) Western Blot showing Kim1 protein levels in miR-21-/- or miR-21+/+ UUO kidneys. (G-J) Morphometric quantification of (G) αSMA stained myofibroblasts (H) endothelial density (J) macrophages. (K) Panels showing low power images of d10 post IRI or sham treated kidneys from miR-21-/- or miR-21+/+ matched mice. (L) Quantification of Sirius red stained fibrosis in kidneys. (M) Q-PCR quantification of Coll1a1 and Coll3a1 transcripts normalized to Gapdh (n = 6-9/group. * P<0.05, **P<0.01 ***P<0.001. Bar = 100μm).