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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Biotech Histochem. 2012 Nov 23;88(8):428–439. doi: 10.3109/10520295.2012.729864

Fig. 1.

Fig. 1

Identification of lymphatic capillaries and mature lymphatic vessels. Paraffin embedded knee sections of a 6-month-old WT mouse stained with anti-podoplanin and anti-αSMA antibodies. Sections were scanned using a x 20 lens and the VS-ASW FL system. Images were observed under x 100–1000 magnification to distinguish lymphatic capillaries and mature lymphatic vessels. A) Left panel: low magnification (x 20) showing the region of interest. Areas that contain lymphatic vessels are outlined. Right panels: enlargements (x 800) of the region indicated in the left image showing a podoplanin+/αSMA-lymphatic capillary (blue arrows). B) Left panel: low magnification (x 20) showing the region of interest. Right panels: enlargements of the region indicated in the left panel showing a podoplanin+/αSMA+ mature lymphatic vessel (white arrows).