Skip to main content
. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Biomol NMR Assign. 2013 Feb 17;8(1):149–153. doi: 10.1007/s12104-013-9472-8

Fig. 1.

Fig. 1

2D 1H-15N- correlation-HSQC spectrum of a 1mM uniformly [1H-15N]-labeled N-HvGRRBP1 sample dissolved in buffer containing 300 mM NaCl, 50 mM Na2HPO4/NaH2PO4, 5 % (v/v) D2O, 0.05% sodium azide, 0.1 mM PMSF, at pH 6.5. The spectrum was acquired at 298 K on a 14.1 Tesla (600 MHz 1H Larmor frequency) Bruker solution NMR spectrometer. NMR signals connected by a thin line correspond to resonances from side chain NH2 groups of glutamine and asparagine residues. NH resonance assignments are indicated by the one-letter code of amino acids and their number position in the protein amino acid sequence on each NMR peak.