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Biotinylation of B2 RNA(81-130) does not affect its ability to potently repress transcription in vitro. B2 RNA(81-130), 5’-biotin-B2 RNA(81-130), or 5’-biotin-B2 RNA(81-115) were individually added to transcription reactions where indicated. 390 nt transcript was monitored. The values indicate the amount of transcript produced normalized to the reaction that did not contain ncRNA.
