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. Author manuscript; available in PMC: 2014 Oct 9.
Published in final edited form as: J Mol Biol. 2013 Feb 8;425(19):3625–3638. doi: 10.1016/j.jmb.2013.01.035

Figure 4.

Figure 4

Properties of Pol II peptides isolated using the approach shown in Figure 3 are highly reproducible. (A) The elution times for peptides identified in the two crosslinking experiments were highly reproducible. The times at which peptides eluted from the column prior to ionization for the two B2 RNA(81-130) crosslinked samples were plotted against one another.

(B) Comparison of the total XIC values of the peptides crosslinked to B2 RNA(81-130) from two separate experiments. The total XIC value for each peptide was normalized by the sum of all XIC values from that sample, and the log of these numbers were plotted. (C) Comparison of the total XIC values of the peptides from two separate trypsin treated Pol II control samples. The total XIC value for each peptide was normalized by the sum of all XIC values from that sample, and the log of these numbers were plotted.