Table 1. Primers used for PCR amplification and sequencing.
Primers for PCR amplification of MYOC gene and Sanger sequencing. One pair of primers was used for promoter region and Exon 1 including exon-intron junction, two pairs of primers were used for Exon 3 including exon-intron junctions. Four sets of primers used for haplotype analysis by genotyping SNPs are shown with respective SNP rs numbers. The Amplicon sizes of amplicons were are shown in base pairs (bp).
| Name | Sequence (5′ – 3′) | Target | Amplicon Size (bp) |
|---|---|---|---|
| M1.1F | GGTGCATAAATTGGGATGTTC | Promoter & Exon 1 | 999 |
| M1.1R | TTGTGCTAGCTGTGCAGTCTC | ||
| M3-3F | TGCGATAACTGAGGCGTAGAG | Exon 3 (Part 1) | 997 |
| M3-3R | GGAGGCTTTTCACATCTTGG | ||
| M3-2F | CATTGACTTGGCTGTGGATG | Exon 3 (Part 2) | 992 |
| M3.1R | CATCCTGCAATCACATCTCC | ||
| MS5F | CTCAATGAGTTTGCAGAGTG | rs235920 | 399 |
| MS5R | TCTGCTGTGCTGAGAGGTG | ||
| MS89F | TGAACCTTTGCTCAGATTG | rs235918 | 399 |
| MS89R | CCACTTTCACAAAAAGTGAC | ||
| MS4F | GTCCTGAACACCTGAGAATC | rs235868 | 396 |
| MS4R | GTAAGCAGGTTTAGGATTGG | ||
| MS2F | CTCAGGCCCAACTGTTATC | rs603930 | 295 |
| MS2R | AGAATTAGCTGGATGTGGTG |