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. Author manuscript; available in PMC: 2014 Jun 1.
Published in final edited form as: Arthritis Rheum. 2013 Jun;65(6):1561–1568. doi: 10.1002/art.37922

Figure 3.

Figure 3

Effects of EGF and fibronectin fragment (Fnf) on MMP-13 production. (A), Fnf increased MMP-13 production as determined by immunoblotting of conditioned media. MMP-13 production was enhanced by co-stimulation with 10ng/mL EGF. EGF alone did not cause any increase in MMP-13. MMP-2 was used as a loading control. (B), Adenoviral overexpression of dominant negative (DN) Rac1 decreased Fnf stimulated MMP-13 production and production in response to EGF + Fnf as determined by immunoblotting of conditioned media. Control wells received null control adenovirus. Cell lysates were immunoblotted for Rac and for actin as a loading control to demonstrate overexpression of Rac DN.