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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Free Radic Biol Med. 2012 Oct 23;53(12):10.1016/j.freeradbiomed.2012.10.542. doi: 10.1016/j.freeradbiomed.2012.10.542

Fig. 4.

Fig. 4

Chloride-dependent HOCl production in the isolated phagosomes. The isolated phagosomes were resuspended in the relaxation buffer with various chloride concentrations. Oxidant production was reconstituted by supplementing NADPH and detected with R19-S, a HOCl sensor. The samples were analyzed for fluorescence intensity change by flow cytometry by gating on the phagosome population. When indicated, the NADPH oxidase inhibitor DPI or the MPO inhibitor ABAH was used to validate HOCl production.