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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Free Radic Biol Med. 2012 Oct 23;53(12):10.1016/j.freeradbiomed.2012.10.542. doi: 10.1016/j.freeradbiomed.2012.10.542

Fig. 7.

Fig. 7

Acidification of PM-PLS is dependent on chloride, which is suppressed by an active NADPH oxidase. PM-PLS were isolated and assayed in the presence of either (A) chloride-free (K gluconate) or (B) chloride-rich (KCl) relaxation buffer containing ATP and AO, as described in the legends of Figs. 3 and 6. After establishment of a stable fluorescence baseline, Sp-cAMP-S was added as indicated by the arrow. As can be seen in (B), in the presence of extraluminal chloride rapid acidification occurred in the absence of NADPH addition but was abolished when NADPH was added to activate the oxidase. Little acidification was observed in the presence or absence of NADPH when the experiment was performed in the chloride-free medium (A).