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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Dev Comp Immunol. 2013 Apr 6;41(1):33–43. doi: 10.1016/j.dci.2013.03.020

Table 2.

Germline gene usage of equine immunoglobulin heavy chain VDJ sequences.

IGHV VD IGHD DJ IGHJ
Sequence gene Identity1 junction gene Identity1 junction gene Identity1
A. Sequences obtained with RACE protocol
FETUS:
IGVDJ1 2S3 98 12 18S1 100 2 1S3 100
IGVDJ2 2S3 98 3 17S1/2 100 0 1S5 97
IGVDJ3 2S3 98 10 18S1 100 0 1S3 100
IGVDJ4 2S3 98 0 9S1 73 3 1S3 100
IGVDJ5 2S3 98 9 18S1 100 0 1S5 100
IGVDJ6 2S3 98 6 22S1 89 0 1S3 100
IGVDJ7 2S3 98 5 15S2 100 0 1S2 100
IGVDJ8 2S3 98 4 13S1 100 0 1S2 100
IGVDJ9 2S3 97 9 24S1 100 0 1S5 93
IGVDJ10 2S3 97 0 15S2 100 0 1S2 100
IGVDJ11 2S3 99 10 15S2 100 7 1S5 100
IGVDJ12 2S3 99 13 22S1 100 0 1S3 100
IGVDJ13 2S2 100 15 18S1 100 2 1S7 100
IGVDJ14 2S2 100 3 18S1 100 6 1S5 100
IGVDJ15 2S2 100 9 7S2 100 7 1S5 100
IGVDJ16 2S2 100 4 11S1 100 0 1S5 100
IGVDJ17 2S2 100 ND IGVDJ108, −115 ND ND 1S3 100
IGVDJ18 2S2 100 6 15S2 100 4 1S6 100
IGVDJ19 2S2 99 1 15S1 100 0 1S4 88
IGVDJ20 2S2 99 6 18S1 93 2 1S3 100
IGVDJ21 2S4 99 11 11S1 100 0 1S5 100
IGVDJ22 2S4 99 8 15S2 100 0 1S3 93
IGVDJ23 2S4 99 8 17S1/2 100 0 1S2 100
IGVDJ24 2S4 98 8 7S2 100 0 1S6 100
IGVDJ25 2S4 98 8 17S1/2 100 0 1S4 100
IGVDJ26 4S2 100 7 18S1 100 0 1S5 100
IGVDJ27 4S2 100 10 18S1 93 5 1S6 100
IGVDJ28 1S3 100 2 18S1 100 9 1S6 100
IGVDJ29 4S17 99 9 7S1 100 0 1S3 92
IGVDJ30 4S17 99 12 18S1 100 5 1S6 100
IGVDJ31 4S5 100 2 4S1 100 0 1S5 100
NEONATE:
IGVDJ32 2S3 96 13 4S1 100 18 1S3 91
IGVDJ33 2S3 97 22 18S1 100 2 1S2 97
IGVDJ34 2S3 97 ND ND ND ND 1S3 100
IGVDJ35 2S3 98 5 15S1 100 17 1S5 97
IGVDJ36 2S3 99 10 15S2 100 14 1S5 100
IGVDJ37 2S3 97 8 4S1 91 0 1S5 88
IGVDJ38 2S3 97 16 15S1 100 12 1S7 85
IGVDJ39 2S3 97 12 15S1 95 0 1S2 73
IGVDJ40 2S3 97 14 10S1 93 4 1S5 93
IGVDJ41 2S3 96 ND ND ND ND 1S5 93
IGVDJ42 2S3 97 3 10S1 100 0 1S7 87
IGVDJ43 2S3 96 11 20S1 100 22 1S3 100
IGVDJ44 2S3 97 24 18S1 100 15 1S5 100
IGVDJ45 2S3 97 10 22S1 100 0 1S5 100
IGVDJ46 2S3 96 2 7S1 88 5 1S5 89
IGVDJ47 2S2 99 16 7S2 100 11 1S7 91
IGVDJ48 2S2 99 12 18S1 100 0 1S2 100
IGVDJ49 2S2 100 4 11S1 100 0 1S5 100
IGVDJ50 2S2 97 14 18S1 93 0 1S5 91
IGVDJ51 2S2 95 12 9S1 94 0 1S2 95
IGVDJ52 2S2 97 20 11S1 100 5 1S7 87
IGVDJ53 2S2 97 ND ND ND ND 1S3 96
IGVDJ54 2S2 98 7 18S1 100 4 1S7 92
IGVDJ55 2S2 98 11 9S1 100 16 1S5 90
IGVDJ56 2S4 100 10 17S1/2 100 0 1S5 97
IGVDJ57 2S4 99 25 4S1 100 6 1S5 93
IGVDJ58 2S4 98 16 6S1 100 10 1S7 100
IGVDJ59 2S4 97 11 15S2 100 0 1S4 100
IGVDJ60 2S4 97 12 9S1 100 7 1S3 95
IGVDJ61 2S4 95 7 26S1 94 0 1S5 97
IGVDJ62 1S6 100 10 7S1 100 8 1S3 96
FOAL:
IGVDJ63 2S3 98 20 26S1 100 2 1S5 97
IGVDJ64 2S3 97 8 14S1 100 0 1S5 93
IGVDJ65 2S3 97 11 17S1/2 100 0 1S5 93
IGVDJ66 2S3 95 24 18S1 100 5 1S5 100
IGVDJ67 2S3 97 5 20S1 100 15 1S3 100
IGVDJ68 2S3 95 26 18S1 100 0 1S6 96
IGVDJ69 2S3 95 ND ND ND ND 1S3 93
IGVDJ70 2S3 97 11 18S1 93 0 1S5 84
IGVDJ71 2S3 97 34 15S2 100 0 1S5 95
IGVDJ72 2S3 92 ND ND ND ND 1S3 80
IGVDJ73 2S3 99 18 26S1 100 6 1S3 100
IGVDJ74 2S3 97 11 18S1 100 5 1S6 100
IGVDJ75 2S3 98 15 7S1 100 6 1S5 100
IGVDJ76 2S3 96 2 20S2 100 6 1S7 83
IGVDJ77 2S3 96 20 17S1/2 93 4 1S3 100
IGVDJ78 2S3 97 23 17S1/2 100 6 1S5 100
IGVDJ79 2S3 96 4 17S1/2 93 6 1S3 100
IGVDJ80 2S3 97 10 18S1 93 12 1S5 100
IGVDJ81 2S3 97 11 17S1/2 93 8 1S5 100
IGVDJ82 2S2 100 7 7S2 100 8 1S5 100
IGVDJ83 2S2 98 8 12S1 85 9 1S5 100
IGVDJ84 2S2 100 10 4S1 94 5 1S3 96
IGVDJ85 2S2 99 11 18S1 100 0 1S2 94
IGVDJ86 2S4 96 13 7S1 100 11 1S3 100
IGVDJ87 2S4 94 4 15S2 93 3 1S5 90
IGVDJ88 2S4 96 ND ND ND ND 1S5 100
IGVDJ89 4S2 100 17 18S1 100 1 1S5 91
IGVDJ90 1S3 100 17 22S1 100 0 1S3 100
ADULT:
IGVDJ91 2S3 97 2 17S1/2 100 6 1S5 74
IGVDJ92 2S3 97 15 2S1 100 19 1S2 100
IGVDJ93 2S3 92 29 4S1 100 17 1S5 85
IGVDJ94 2S3 89 0 7S1 100 20 1S5 90
IGVDJ95 2S3 92 23 2S1 100 0 1S5 79
IGVDJ96 2S3 90 16 10S1 100 9 1S5 89
IGVDJ97 2S3 91 13 28S1 100 1 1S5 100
IGVDJ98 2S3 93 16 15S2 92 14 1S5 82
IGVDJ99 2S3 89 20 5S3 100 21 1S7 81
IGVDJ100 2S3 91 17 6S1 100 6 1S5 85
IGVDJ101 2S3 93 ND ND ND ND 1S5 94
IGVDJ102 2S3 90 10 9S1 94 15 1S3 100
IGVDJ103 2S3 87 37 16S1 100 3 1S2 100
IGVDJ104 2S3 92 18 2S1 100 2 1S5 96
IGVDJ105 2S3 90 22 1S1 100 13 1S5 93
IGVDJ106 2S3 93 8 20S1 100 17 1S5 67
IGVDJ107 2S3 87 9 15S2 91 0 1S5 67
IGVDJ108 2S3 87 0 IGVDJ17, −115 100 28 1S3 88
IGVDJ109 2S3 87 12 22S1 100 17 1S4 74
IGVDJ110 2S3 91 37 18S1 100 0 1S2 91
IGVDJ111 2S3 91 16 16S1 100 1 1S5 77
IGVDJ112 2S3 83 ND ND ND ND 1S3 100
IGVDJ113 2S2 92 1 26S1 100 9 1S5 75
IGVDJ114 2S2 92 30 25S1 93 4 1S2 97
IGVDJ115 2S2 89 ND IGVDJ17, −108 ND ND 1S3 91
IGVDJ116 2S4 93 6 18S1 100 11 1S3 93
IGVDJ117 2S4 89 23 18S1 100 3 1S5 100
IGVDJ118 2S4 86 30 14S1 100 0 1S3 82
IGVDJ119 2S4 89 14 12S1 100 4 1S3 96
IGVDJ120 2S3 86 14 15S1 100 9 1S4 86
IGVDJ121 4S2 84 19 22S1 94 4 1S5 85
B. Sequences obtained with RT-PCR protocol
Fet Spln VDJ1 2S3 99 9 11S1 100 1 1S5 100
Fet Spln VDJ2 2S3 99 6 4S1 100 0 1S3 100
Fet Spln VDJ3 2S2 100 0 11S1 100 0 1S5 98
Fet Spln VDJ4 2S2 100 2 15S2 100 6 1S5 98
Fet Spln VDJ5 2S2 100 0 17S1/2 96 0 1S5 100
Fet Spln VDJ6 2S3 97 ND ND ND ND 1S3 100
Fet Spln VDJ7 2S4 98 5 20S1 94 0 1S3 100
Fet Spln VDJ8 2S3 97 1 18S1 100 4 1S6 100
Fet Spln VDJ9 2S3 96 ND ND ND ND 1S5 100
Neo MLN VDJ1 2S4 97 ND ND ND ND 1S7 88
Neo MLN VDJ2 2S3 97 11 19S1 100 0 1S5 98
Neo MLN VDJ3 2S3 100 7 7S1 100 2 1S3 93
Neo MLN VDJ4 2S2 99 0 17S1/2 83 8 1S5 95
Neo MLN VDJ5 2S2 98 5 15S2 100 4 1S2 96
Neo MLN VDJ6 2S2 100 ND ND ND ND 1S5 96
Neo MLN VDJ7 2S3 or 2S4 95 12 15S1 86 1 1S5 90
Neo MLN VDJ8 2S2 99 3 15S2 100 8 1S5 96
Neo MLN VDJ9 2S2 97 2 13S1 86 0 1S5 91
Foal MLN VDJ1 2S3 97 10 4S1 93 4 1S3 100
Foal MLN VDJ2 2S2 99 5 10S1 95 15 1S5 100
Foal MLN VDJ3 2S3 97 7 4S1 94 1 1S5 100
Foal MLN VDJ4 2S4 99 6 18S1 92 0 1S3 97
Foal MLN VDJ5 2S3 97 3 18S1 100 1 1S5 100
Foal MLN VDJ6 2S4 97 12 7S2 100 3 1S7 100
Foal MLN VDJ7 2S3 96 ND ND ND ND 1S5 100
Foal MLN VDJ8 4S1 99 ND ND ND ND 1S5 100
Foal MLN VDJ9 4S2 99 ND ND ND ND 1S5 97
Adult MLN VDJ1 2S3 94 ND ND ND ND 1S5 92
Adult MLN VDJ2 2S3 70 ND ND ND ND 1S3 92
Adult MLN VDJ3 2S3 87 ND ND ND ND 1S5 91
Adult MLN VDJ4 2S4 78 ND ND ND ND 1S5 94
Adult MLN VDJ5 2S4 78 ND ND ND ND ND ND
Adult MLN VDJ6 2S2 90 ND ND ND ND 1S2 94
Adult MLN VDJ7 2S4 92 ND ND ND ND 1S5 97
Adult MLN VDJ8 2S4 92 ND ND ND ND 1S2 100
Adult MLN VDJ9 2S2 93 ND ND ND ND 1S5 97

‘ND’ denotes germline gene segment could not be determined

1

Identity indicates the percent nucleotide identity of the sequence to the germline gene