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. Author manuscript; available in PMC: 2013 Jun 4.
Published in final edited form as: Nat Biotechnol. 2012 May 27;30(6):531–542. doi: 10.1038/nbt.2239

Figure 7.

Figure 7

Similarities between hESC- and hiPSC-derived progenitors and their suggested correspondence to pre- and gastrulation-stage mouse embryonic precursors. (a) Flow cytometry analyses of CXCR4, ROR2, CD87 and APA expression in dissociated hESCs (red) and hiPSCs (green) revealed similar-sized populations in both sources (top). Gray lines represent isotype controls. Bottom: mRNA expression fold-change profiles in CXCR4+, ROR2+, CD87+ and APA+ populations versus the respective negative populations sorted from hESCs (red) and hiPSCs (green). CXCR4+ cells were isolated from CM-treated cultures, while ROR2, CD87, and APA cells were isolated from BMP4-treated cultures. Analysis is based on two experiments conduced with cells at different passages. (b) Proposed correspondence of progenitor groups no. 1–4 to E5.5 (left) and E7.5 (right) mouse embryos. The human cell surface markers of each progenitor group are listed.