Fig. 4.

Electrophoretic karyotyping of nine clinical isolates of C. glabrata with small chromosomes. S. cerevisiae S288C (Y1307) and CBS 138 were included as references to determine the size of the new chromosomes. Y624a is a daughter strain of KA000127 (Y624) which has lost its small chromosome but the position of the small chromosome, as it would be in Y624, is circled. Y624 and its small chromosome were described previously (Poláková et al. 2009). The sizes of small chromosomes determined by calculation of chromosomal migration on the gel were estimated to be between 280 and 420 kb (see also Table 2). Note, strains Y1640 and Y1641 are from the same patient taken at different time points and the two small chromosomes have a slightly different size