Fig. 5.

Chromosome separations (a and c) and Southern blots (b and d) of C. glabrata clinical isolates with small chromosomes. CBS 138 was used as a reference. The gel a was transferred to membrane b, which was hybridized with the “Gel” gene probe originating from CBS 138 chromosome G. The Y1640 and Y1641 small chromosomes (arrowed) hybridized to the gel probe showing that they share the origin with chromosome G. Note that in these two strains only one signal was obtained. The chromosomes from gel c were transferred to membrane d and hybridized with the probe “Dcl” (originating from chromosome D). Note that in both Y1642 and Y1644 there were two bands, the original chromosome and the small chromosome, hybridizing to the probe