Table 2.
Comparison between CK19 RT-qPCR, multiplex RT-qPCR and AdnaTest for the detection of CTC in breast cancer
| Molecular assay | CK19 RT-qPCR | Multiplex- RT-qPCR | Total | ||
|---|---|---|---|---|---|
| Early breast cancer, (number = 254) | |||||
| AdnaTest | Positive | Negative | Positive | Negative | Total (%) |
| Positive | 4 | 38 | 5 | 37 | 42 (16.5) |
| Negative | 32 | 180 | 53 | 159 | 212 (83.5) |
| Total (%) | 36 (14.2) | 218 (85.8) | 58 (22.8) | 196 (77.2) | 254 |
| Kappa test | poor agreement: Kappa = -0.059 (P = 0.344) | poor agreement: Kapp = -0.114 (P = 0.065) | |||
| Concordance (%) | 184 (72.4), Pa = 0.344 | 164 (64.6), Pa = 0.065 | |||
| Verified metastasis, (number = 51) | |||||
| AdnaTest | Positive | Negative | Positive | Negative | Total (%) |
| Positive | 17 | 11 | 16 | 12 | 28 (54.9) |
| Negative | 4 | 19 | 4 | 19 | 23 (45.1) |
| Total (%) | 21 (41.2) | 30 (58.8) | 20 (39.2) | 31 (60.8) | 51 |
| Kappa test | Moderate agreement: Kappa = 0.422 (P = 0.002), | Fair agreement: Kappa = 0.386 (P = 0.004) | |||
| Concordance (%) | 36 (70.6), Pa = 0.002 | 35 (68.6), Pa = 0.004 | |||
achi-square test, kappa test; 95% CI (0.504, 0.848). The same result was found when we used the chi-square test (Table 2).