Table 3.
Comparison between CK19 RT-qPCR, multiplex RT-qPCR and AdnaTest for the molecular characterization of CTC in breast cancer.
| Multiplex RT-qPCR | Singleplex RT-qPCR | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| AdnaTest/ | CK19 | HER-2 | MAGE A3 | CK19 | |||||
| Pos. | Neg. | Pos. | Neg. | Pos. | Neg. | Pos. | Neg. | ||
| Early breast cancer, (number = 254) | |||||||||
| GA733-2 | Positive | 1 | 8 | 0 | 9 | 0 | 9 | 2 | 7 |
| Negative | 24 | 221 | 28 | 217 | 12 | 233 | 34 | 211 | |
| Concordance (%) | 87.4 | Pa = 0.897 | 85.4 | Pa = 0.284 | 91.7 | Pa = 0.496 | 83.9 | Pa = 0.481 | |
| MUC-1 | Positive | 0 | 14 | 1 | 13 | 0 | 14 | 1 | 13 |
| Negative | 24 | 215 | 27 | 213 | 12 | 228 | 35 | 205 | |
| Concordance (%) | 84.6 | Pa = 0.213 | 84.3 | Pa = 0.633 | 89.8 | Pa = 0.391 | 81.1 | Pa = 0.438 | |
| HER-2 | Positive | 2 | 24 | 2 | 24 | 0 | 26 | 2 | 24 |
| Negative | 23 | 205 | 26 | 202 | 12 | 216 | 34 | 194 | |
| Concordance (%) | 81.5 | Pa = 0.698 | 80.3 | Pa = 0.567 | 85 | Pa = 0.231 | 77.2 | Pa = 0.317 | |
| Verified metastasis, (number = 51) | |||||||||
| GA733-2 | Positive | 14 | 6 | 6 | 14 | 3 | 17 | 15 | 5 |
| Negative | 4 | 27 | 2 | 29 | 1 | 30 | 6 | 25 | |
| Concordance (%) | 80.4 | Pb < 0.001 | 68.6 | Pb = 0.045 | 64.7 | Pb = 0.287 | 78.4 | Pb = 0.000 | |
| MUC-1 | Positive | 14 | 9 | 6 | 17 | 3 | 20 | 14 | 9 |
| Negative | 4 | 24 | 2 | 26 | 1 | 27 | 7 | 21 | |
| Concordance (%) | 74.5 | Pb = 0.001 | 62.7 | Pb = 0.119 | 58.8 | Pb = 0.316 | 68.6 | Pb = 0.012 | |
| HER-2 | Positive | 11 | 7 | 5 | 13 | 1 | 17 | 13 | 5 |
| Negative | 7 | 26 | 3 | 30 | 3 | 30 | 8 | 25 | |
| Concordance (%) | 72.6 | Pb = 0.006 | 68.6 | Pb = 0.112 | 60.8 | Pb = 1 | 74.5 | Pb = 0.001 | |
achi-square test; bFisher's exact test; P < 0.05 is considered as significant.