Table 1.

List of primers used in this study

PCR	Primer	Sequence (5'-3')	Product size (bp)	PCR cycles	Annealing temperature (°C)
MSP	DACT1m3	CGGGATAGTAGTAGTCGGC	118	41	60
	DACT1m4	CGCTAAAACTACGACCGCG
	DACT1u3	GTTGGGATAGTAGTAGTTGGT	123	41	58
	DACT1u4	AAACACTAAAACTACAACCACA
RT-PCR	DACT1-F	AGGAGAAGTTCTTGGAGGAG	179	32	55
	DACT1-R	TGAGCTAGGCCGACTGTCTG
	GAPDH-F	GGAGTCAACGGATTTGGT	206	23	55
	GAPDH-R	GTGATGGGATTTCCATTGAT
Real-time PCR	DACT1-F	GACGAGCAGAGCAATTACACC	158	40	60
	DACT1-R	ACCGTTTGAATGGGCAGA
	β-actin-F	CCTGTGGCATCCACGAAACT	314	40	60
	β-actin-R	GAAGCATTTGCGGTGGACGAT