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. 2013 Apr 8;15(2):R49. doi: 10.1186/ar4208

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Copyright © 2013 Lantow et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Differential response of B6.Sle2c2 and B6 leukocytes to granulocyte-colony stimulation factor (G-CSF). (A) Mouse (m)G-CSF binding by B6 and B6.Sle2c2 leukocytes measured with biotinylated G-CSF and steptavidin-PE (G-CSF-biot-PE). The top row shows representative overlays of the B6 (black) and Sle2c2 (gray) histograms, with the gray-filled histograms showing the controls without added mG-CSF for the indicated gated cell populations. (B) Total CD11b⁺, GR1^hiCD11b⁺and GR1^loCD11b⁺peripheral blood leukocytes (PBLs) in untreated B6 and B6.Sle2c2 mice (n = 6 per strain), and 4 d after injections of 1.2, 12.0 or 120.0 ug human (hu)G-CSF (n = 2 per dose per strain). The graphs show mean and standard error of the mean with the significance value from the t-test (*P < 0.05; **P < 0.01; ***P < 0.001).