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The biomarker panel including ITIH5, DKK3, and RASSF1A enables significant discrimination of breast cancer sera from various control conditions. Scatterplot illustrating the mean PMR values of the three-gene panel in the independent validation set. Combination of DKK3 and ITIH5 with RASSF1A increased the sensitivity to 67% (92 of 138) with a specificity of 69% (93 of 135) and 82% (32 of 39) in healthy and benign disease controls, respectively. ***P < 0.0001.
