IFN-γ T cell subset responses in MSP1 vaccinated volunteers against peptide pools containing either MSP133 3D7 peptides, MSP133 Wellcome peptides or a combined pool of both allelic peptides. To compare IFN-γ responses from different T cell subsets ‘Undepleted’ PBMC (A), CD4+ (CD8+ depleted) PBMC (B) and CD8+ (CD4+ depleted) PBMC (C) were prepared using a MACS magnetic column depletion kit and responses to peptide pools measured. Graphs represent the individual responses expressed as IFN-γ SFU/106 PBMC from various time-points selected for high responses and PBMC availability (#10, 12 = d14; #1, 9, 14 = d63; #2, 3, 4, 8, 11 = d84; #5 = dC-1; #13 = dC+35; #7 = dC+90; #6 = d140; see Figure S3). Ratios for the 3D7/Well median response are included (>1 = stronger 3D7 response).