. Author manuscript; available in PMC: 2014 Apr 1.

Published in final edited form as: Curr Protoc Immunol. 2013 Apr;0 15:Unit–15.1451. doi: 10.1002/0471142735.im1514s101

Table 15.14.2.

Comparison of Immunization Protocols for Induction of EAM in Mice and Rats

Species	Cardiac myosin source	Antigen dose (μg)^a	CFA supplement^b	Optional adjuvant (dose per animal)^c
Mouse	Mouse or rat	100	+/− CFA	100 nmol B. pertussis toxin (PTX) on day 0
Rat	Rat, pig, or human	500	10 mg/mL	2 × 10⁹ heat-killed B. pertussis on days 1 and 3

Minimum dose per animal. Immunizations are performed on days 0 and 7. Animals are sacrificed on day 21.

M. tuberculosis strain H37Ra.

Administered intraperitoneally.