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Results of the titration of 30 μM annexin a5 with lipid as LUVs made of a 60:40 mixture of POPC/POPS in the presence of 0.75 mM Ca2+ at 15°C. Top: (Above) Raw ITC data. (Below) Heat of dilution data (control). Middle: Integrated heats of binding as a function of ligand/protein ratio. Bottom: Binding isotherm of fractional saturation as a function of free [Lipid].
