Figure 6.

(A) Results of the titration of 24 μM annexin a5 with Ca²⁺ in the presence of 2 mM total lipid as LUVs made of a 60:40 mixture of POPC/POPS at 18°C (solid black circles). Results of the titration of 30 μM annexin a5 with Ca²⁺ in the presence of 4 mM total lipid as LUVs made of a 80:20 mixture of POPC/POPS at 18°C (solid cyan circles). Top: (Upper Frame) Raw ITC data from the titration of 30 μM annexin a5 with Ca²⁺ in the presence of 4 mM total lipid made of a mixture of 80:20 POPC/POPS at 18°C. (Lower Frame) Heat of dilution data (control). Bottom: Integrated heats of binding displayed as a function of ligand/protein ratio of annexin a5 with Ca²⁺ in the presence of 2 mM total lipid made of a mixture of 60:40 POPC/POPS (solid black circles) and in the presence of 4 mM total lipid made of a mixture of 80:20 POPC/POPS (solid cyan circles). (B) Results of the titration of 30 μM annexin a5 with lipid as LUVs made of a 60:40 mixture of POPC/POPS in the presence of 0.75 mM Ca²⁺ (solid black circles) at 15°C. Results of the titration of 20 μM annexin a5 with lipid as LUVs made of a 80:20 mixture of POPC/POPS in the presence of 2 mM Ca²⁺ (solid cyan circles) at 15°C. Top: (Upper Frame) Raw ITC data from the titration of 20 μM annexin a5 with lipid composed of a mixture of 80:20 POPC/POPS in the presence of 2 mM Ca²⁺ at 15°C. (Lower Frame) Heat of dilution data (control). Bottom: Integrated heats of binding displayed as a function of ligand/protein ratio of both annexin a5 with lipid composed of a 60:40 POPC/POPS mixture (solid black circles) and 80:20 POPC/POPS mixture (solid cyan circles) in the presence of 0.75 mM and 2 mM Ca²⁺, respectively.