Figure 7.

Distribution of all possible states of annexin a5 as a function of increasing concentrations of Ca²⁺. Solid line: ligand-free protein; dotted line: Ca²⁺-bound protein; dashed line: lipid-bound protein; dot-dashed line: Ca²⁺ and lipid-bound protein. (Top) Distributions calculated using the binding constants in Table 1 for 24 μM annexin a5 assuming a total lipid concentration of 0.02 mM. (Middle) Distributions similarly calculated for 24 μM annexin a5 assuming a total lipid concentration of 200 mM. (Bottom) Distributions similarly calculated for 24 μM annexin a5 in the presence of 2 mM total lipid.