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Shown is the structure of Dab(T)-TN-(T)Ru, which is typical of the constructs employed here. The quencher, 4-dimethylaminoazobenzene (DABSYL), is attached to the base of the 5′ terminal thymine and the luminophore, a ruthenium tris(bipyridine) variant, is attached to the base of the 3′ terminal thymine. (Thus, all constructs, irrespective of their sequence, begin and end with a thymine.)
