Figure 3.
The end-to-end collision rates of single-stranded poly-thymine and poly-adenine at 22 ± 1°C (295 K) show similar power law dependencies. The rollovers observed at very short polymer lengths reflect the presence of the highly flexible linkers (22). Note that our poly-adenine constructs include a thymine at each end to which the luminophore and quencher are attached; each construct is thus 2 bases longer than the number of internal adenines it contains (for example, the A25 construct is 27 bases long and has the sequence TA25T).