Figure 5.

(a) o-SRCD spectrum of dehydrated MEG-14 deposited on a quartz silica plate. (b–d) The process of rehydration was monitored as a function of time. Initially (bold, black), a huge increase of the band at 200 nm region is noted (bold to dash); (c) At a later time the observed reductions of the intensities of the spectral bands are most likely due to the protein dilution in the hydrated cell (as evidenced by the decrease in the HT signal (not shown)); (d) Finally, there is a decrease of the 222 nm minimum and a shift of the 202 peak to 198 nm, indicating the acquisition of a more disordered conformation in the protein.