Figure 4.

Effects of BPA exposure on serum FSH and LH levels and FSH and LH protein expression in the pituitary glands. Adult female rats were administered BPA (0, 0.001, or 0.1 mg/kg BWU/day) or EB (0.001 mg/kg BW/day) for 90 days by gavage. FSH (A) and LH (B) levels were measured by ELISA; values represent mean ± SD (n = 12). (C) Western blot analysis of FSH and LH in pituitary gland protein extracts. (D) Densitometric quantification of FSH and LH protein levels. Data represent the mean ± SD of at least three independent experiments. (E) Immunohistochemical localization of FSH and LH in the anterior pituitary glands (original magnification: 400×; bar = 30 μm). *p < 0.05, and **p < 0.01 compared with control (0 mg/kg BW).