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. 2013 Mar 19;121(6):663–669. doi: 10.1289/ehp.1205823

Figure 4.

Figure 4

Effects of BPA exposure on serum FSH and LH levels and FSH and LH protein expression in the pituitary glands. Adult female rats were adminis­tered BPA (0, 0.001, or 0.1 mg/kg BWU/day) or EB (0.001 mg/kg BW/day) for 90 days by gavage. FSH (A) and LH (B) levels were measured by ELISA; values represent mean ± SD (n = 12). (C) Western blot analysis of FSH and LH in pituitary gland protein extracts. (D) Densitometric quantification of FSH and LH protein levels. Data represent the mean ± SD of at least three independent experiments. (E) Immunohistochemical localization of FSH and LH in the anterior pitui­tary glands (original magnification: 400×; bar = 30 μm). *p < 0.05, and **p < 0.01 compared with control (0 mg/kg BW).