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. 2013 Apr 19;121(6):717–724. doi: 10.1289/ehp.1206301

Figure 2.

Figure 2

Chemical induction of aneuploidy in C. elegans. (A) Pxol-1::GFP worms were exposed to 100 µm nocodazole or 0.1% DMSO (control) for 24 hr. Two GFP+ embryos are visible within the nocodazole-treated worm’s uterus (arrows) adjacent to the autofluorescence emanating from the gut; bar = 50 µm. (B) Embryonic viability (mean percent ± SE) after either DMSO or nocodazole exposure. (C) Chemotherapeutic screen; the worms were exposed for 24 hr or 65 hr to 100 µM of each compound. The number of GFP+ embryos per worm was recorded, corrected for the average number of embryos found in each worm, and expressed as the log fold ratio over DMSO (mean percent ± SE; two-tailed Mann–Whitney U test, 95% CI, chosen over ANOVA with post-test correction to test for significant differences for each compound over DMSO because of high differences in sample variance). Each chemical exposure was performed six times. aLethal at 65 hr. *p ≤ 0.05, and **p ≤ 0.01, by two-tailed Mann–Whitney U test, 95% CI.