Figure 3.

Screening of environmental chemicals. Worms were exposed for either 24 hr (A) or 65 hr (B) to each compound, at a concentration of 100 µM [except for mancozeb, dicofol, 2-(thiocyanomethylthio) benzothiazole (TCMTB), phosalone, chlorophene, endosulfan, parathion-methyl, which were further diluted 10-fold, and chlorpyrifos-methyl, which was used at 1 µM, to circumvent lethality]. The number of green embryos per worm was recorded and corrected for the average number of embryos found in each worm. The number was then expressed as the log fold ratio over DMSO. Chemicals are listed in order in Supplemental Material, Tables S2 and S3 (http://dx.doi.org/10.1289/ehp.1206301). The compounds were categorized according to their assessed mammalian reproductive toxicity [i.e., the number of mammalian end points for which they were positive: high reproductive toxicity (> 2 end points), intermediate reproductive toxicity (1 end point), and no reproductive toxicity (0 end points)]. At 65 hr, the mean value of fold-induction for the high and intermediate reproductive toxicity groups was significantly higher than the no–reproductive toxicity group (p = 0.008). Each chemical was tested three times.