Figure 5.

High throughput detection of genetic and chemical induction of aneuploidy. (A) Overlay of bright field and fluorescence images of a Pxol-1::GFP, him-8 worm with two embryos; the GFP⁺ embryo is clearly distinguishable from the GFP^– embryo next to it (bar = 100 µm). (B) Automated detection of GFP⁺ embryos using the COPAS BIOSORT; the black line represents the GFP⁺ threshold as determined by Pxol-1::GFP without him-8 (WT count). The GFP⁺ population of embryos is more abundant in nocodazole-treated worms compared with DMSO-treated worms in both the Pxol-1::GFP and the Pxol-1::GFP; col-121 (sensitized) backgrounds.