Figure 3. IL-23 is dispensable for homeostatic maintenance and plasticity of intestinal T_H17.

a) Number of eYFP⁺ T_H17 cells in LN and PP of Il17a^CreR26R^eYFP (B6, closed dots) and Il17a^CreR26R^eYFPIl23a-deficient (Il23a^−/−, open dots) mice. b) Flow cytometry of eYFP⁺ CD4⁺ T cells showing expression of CXCR5 and PD-1 in PP of B6 and Il23a^−/− mice. c) Quantitative PCR analysis for expression of indicated mRNA in FACS purified PP or LN eYFP⁺ CD4⁺ T cells from B6 and Il23a^−/− mice. d) Flow cytometry of eYFP⁺ CD4⁺ T cells from LN or PP of B6 and Il23a^−/− mice showing intracellular staining for IL-17, IFNγ and IL-22. Data are representative of at least three independent experiments. *, p-value < 0.01.