Fig.6. Cholera toxin specific IgA response in Il17a^Cre R26R^eYFP mice.

a) Proportion of eYFP⁺ cells in PP CD4⁺ T cells (left panel), proportion of CXCR5⁺ PD-1^high cells in eYFP⁺ CD4⁺ T cells (middle panel) and ELISA quantification of cholera toxin-specific IgA (right panel) in cholera toxin-immunized Il17a^CreR26R^eYFP mice 7-10 days after challenge. b) Quantitative PCR analysis for expression of indicated mRNA in FACS purified eYFP⁺ and eYFP⁻ CD4⁺ T cells from PP of cholera toxin-immunized Il17a^CreR26R^eYFP mice 10 days after challenge. mRNA expression is relative to Hprt. Data in a and b are representative of three independent experiments. c) Flow cytometry of eYFP⁺ or eYFP⁻ CD4⁺ TCRβ⁺ T (shaded line) and T_FH (solid line) cells from PP of cholera toxin-immunized Il17a^CreR26R^eYFP mice 10 days after challenge. d) MFI values of cell types as in c). Mean values +/− SEM are shown for three individual mice. *, p-value < 0.05; **, p-value < 0.005.