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. Author manuscript; available in PMC: 2013 Nov 1.
Published in final edited form as: Nat Immunol. 2013 Mar 24;14(5):500–508. doi: 10.1038/ni.2556

Figure 4. Slc7a5fl/flCD4-Cre mice.

Figure 4

(a) PCR analysis of genomic DNA isolated from the indicate thymocytes showing Slc7a5 floxed (FL), wild-type (WT) and deleted (DEL) PCR products. (b) Thymocyte (left) and thymocyte subset (middle) numbers of Slc7a5fl/fl and Slc7a5fl/flCD4-Cre mice, the right panel shows flow cytometric analysis of NKT cells from the indicated mice (c) CD98 in thymic subsets. (d,e) Cellular analysis of spleen and lymph node from Slc7a5fl/fl and Slc7a5fl/flCD4-Cre mice. (d) Flow cytometry analysis of CD98, CD62L and CD44 levels in lymph node T cells. (e) Total numbers of spleen (left) and brachial lymph node cells (center left). Flow cytometry analysis of CD4 and CD8 expression by lymph node T cells (center right) or Foxp3 and CD25 expression by splenic T cells (right). (f) immunoblot analysis with Slc7a5 antibodies of naïve and 20 h TCR stimulated Slc7a5fl/fl and Slc7a5fl/flCD4-Cre CD8+ T cells. (g) 3H-phenylalanine uptake by Slc7a5fl/fl and Slc7a5fl/flCD4-Cre CD8+ TCR stimulated (20 h) T cells, p=0.0003. Uptake performed in the presence or absence of cold competitor 10 mM Leu to quench. (b-e). Representative data from 8-12 week mice, 3 mice per group; (f) representative of 3 experiments. SMC1 is loading control. (g) 3 mice per group.