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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Nat Immunol. 2013 Mar 3;14(4):327–336. doi: 10.1038/ni.2548

Figure 3. TRIM21 signaling is dependent upon TAK1 and stimulates κB, AP-1 and IRF pathways.

Figure 3

(a) NF-κB luciferase induction in wild-type MEF cells when treated with DMSO or inhibitors 7 h post challenge with AdV, Ab or AdV + Ab. (b) ELISA showing induction of phosphorylated (p-) and total IKKα (Ser176 and Ser180) and p65 (Ser536) 4 hours post-challenge of wild-type (WT) or Trim21-deficient (K21) MEF cells. (c) DNA binding ELISA showing of induction of AP-1 components binding to consensus oligonucleotides 4 h post challenge of Trim21-deficient MEF cells transduced with empty vector (K21 EV) or human TRIM21 (K21 T21). (d) ELISA showing phosphorylated (Ser73) and total c-Jun induction after treatment with indicated stimuli in Trim21-deficient MEF cells or Trim21-deficient MEF cells transduced with human TRIM21. (e) DNA binding ELISA showing induction of IRF3, IRF5, IRF7 and IRF8 binding to DNA response elements 4 h post-infection in wild-type and Trim21-deficient MEF cells. For all panels, error bars represent SEM from three replicates.