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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2013 Mar 4;190(7):3246–3255. doi: 10.4049/jimmunol.1201484

Figure 1.

Figure 1

The importance of IL-23 for differentiation of human Th17 responses. A: Naïve human CD4+ CD45RA+ T-cells were purified from peripheral blood. Typical purity was >95% CD4+CD45RA+, data are representative of 8 independent donors. B: Naïve CD4+CD45RA+ T-cells were co-stimulated for 5d with anti-CD28 in the presence of recombinant cytokines. After resting for 2d, cells were re-stimulated with anti-CD3 and IL-17A release determined by ELISA (2 representative donors from 11 experiments are shown). C: Summary plot showing the effect of addition of IL-23 to IL-1, IL-6 primed Th17 responses from all 11 independent experiments. In all graphs, *:0.05>p value≥0.01, **:0.01>p value≥0.001, ***: p value<0.001.