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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: J Immunol. 2013 Mar 4;190(7):3246–3255. doi: 10.4049/jimmunol.1201484

Figure 4.

Figure 4

X-rays activate ATM in DC resulting in depressed IL-23. A: Ionising radiation activates ATM in DC. ATM-inhibited or competent moDC were exposed to the conditions including LPS and X-rays and phospho-ATM expression determined by Western blot (representative donor of 3). B: The phosphorylation status of ATM was further confirmed by intracellular staining. DC were exposed to 6Gy of X-rays +/− pretreatment with KU55933. The phosphorylation of ATM was examined by indirect intracellular staining with a phospho-ATM-specific antibody, dotted line: iDC, dashed line: X-ray exposed DC, thick solid line: KU55933 pretreated DC after X-ray exposure. C: Representative data from moDC of 1 of 6 donors exposed to KU55933 (10μM) or left un-treated prior to exposure to X-rays. Cells were subsequently activated with LPS/IFNγ and IL-23 secretion determined by ELISA. Error bars indicate the SD of triplicate biological determinations. D: Summary of 6 donors showing that ATM-inhibition negates the inhibition of IL-23 following exposure to X-rays (6Gy). Line indicates mean response.