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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Immunol. 2012 Nov 23;190(1):381–391. doi: 10.4049/jimmunol.1201330

Figure 1. Incorporating a PH domain point mutation does not affect ARAP3 expression.

Figure 1

(A) Protein from 1×106 bone marrow derived neutrophils from control (Arap3+/+), heterozygous (Arap3+/PH*) or homozygous mutant (Arap3PH*/PH*) mice was immunoblotted for ARAP3 (top panel) or β-COP (bottom panel) as a loading control. Representative experiment shown from four independent experiments. (B) Peripheral blood cells from bone marrow chimeras reconstituted with Arap3PH*/PH* (PH*) or matched control (WT) hematopoietic stem cells were analysed using a Vet ABC animal blood cell counter. Data shown (means ±SEM) were obtained with 14 wild-type and 12 knock-in chimeras generated with three individual bone marrow donors per genotype.