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. Author manuscript; available in PMC: 2013 Sep 15.
Published in final edited form as: Cancer Res. 2013 Mar 6;73(6):1800–1810. doi: 10.1158/0008-5472.CAN-12-1916

Figure 2. Reintroduction of Arkadia in NCI-H460 cells restores TGF-β-dependent Ski/SnoN degradation, transcriptional responses and inhibits anchorage independent growth, but has no effect on their tumorigenic properties in vivo.

Figure 2

A. Western blot showing Arkadia levels in two NCI-H460-derived cell lines expressing FLAG-tagged Arkadia. Cells were treated for 4 h ± MG132. Actin is a loading control. B. Whole cell extracts from cells treated ± TGF-β Western blotted using the antibodies indicated. C. Luciferase assays were performed as in Fig. 1. D. Whole cell extracts prepared from the cell lines indicated and Western blotted using antibodies shown. E. NCI-H460 parental cells or the clones expressing FLAG-tagged Arkadia were grown in soft agar for 10 days. The numbers of colonies were quantified and are shown in the bar graph (right). F. The cell lines shown were injected subcutaneously in both flanks of Balbc nu/nu mice. The data are the averages of the tumor volumes for four mice (8 tumors in total) per cell line.