Figure 5. The ability of MDA-MB-231 cells to colonize lungs requires Arkadia function.

A–C. GFP and mCherry-labeled MDA-MB-231 cells were transfected with a control (NT), and GFP-labeled MDA-MB-231 cells were transfected with two different siRNAs against Arkadia (Ark #1 and #2). 24 h later cells were split for immunoblots, CAGA₁₂-Luciferase reporter assays and mice experiments. A. Whole cell extracts were prepared from the GFP-labeled cells treated ± TGF-β and Western blotted using the antibodies indicated. B. The GFP-labeled cells were transfected with the CAGA₁₂-Luciferase and TK-Renilla reporters then treated ± TGF-β. Coupled Luciferase/Renilla assays were as in Fig. 1. C. Equal numbers of NT mCherry cells were mixed with either NT GFP cells or Ark #1 and Ark #2 siRNA GFP cells and were injected into the tail vein of immunodeficient mice. Analysis and quantitation of the experiment was as in Fig. 4.