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The role of the agmatidine modification in decoding. A) Comparison of the A3•agm2C wobble pair with a canonical G3-C34 Watson-Crick base pair (grey) and an A3•C34 mismatch (cyan), both observed in the wobble position 12. B) Interaction of the terminal amine of the agmatidine modification on the A-site tRNA with the backbone of a downstream mRNA residue important for stabilizing the codon-anticodon interaction.
