Table 1.
2D arrayed proteins identified by LC ESI MS/MS
| Protein | Accession numbera |
2D unmodified gel spot numberb |
2D AGE BM gel spot numberc |
Score | Coverage (%) |
Calculated molecular mass (Da)d |
Pl d | Fold change to control matrix (n=3) |
Up/down regulation |
Previously identified ref. no. |
|---|---|---|---|---|---|---|---|---|---|---|
| Protein disulphide isomerase | P30101 | 1 | 5 | 11.6 | 30.7 | 56746 | 5.98 | 2.08 | U | |
| Gamma enolase | P09104 | 2 | 45 | 11.6 | 58.2 | 47108 | 4.91 | 0.96 | N | 25 - |
| Phosphoglycerate kinase | P00558 | 4 | 46 | 11.6 | 44.8 | 44568 | 8.52 | 8.37 | D | 25 - |
| c myc promoter binding protein | P22712 | 5 | 47 | 11.6 | 11.0 | 37063 | 7.23 | 4.86 | D | 25 - |
| c myc promoter binding protein | P22712 | 6 | 48 | 11.6 | 11.0 | 37063 | 7.23 | 4.14 | D | 25 - |
| Cathepsin D precursor | P07339 | 3 | 8 | 11.6 | 2.2 | 44523 | 6.1 | 1.30 | N | 25 26 |
| Cathepsin D precursor | P07339 | 10 | 9 | 11.6 | 10.9 | 44523 | 6.1 | 0.71 | N | 25 26 |
| Triosephosphate isomerase | P60174 | 12 | 14 | 11.6 | 63.7 | 26521 | 6.51 | 2.64 | D | –26 |
| Heat shock protein beta-1 | P04792 | 14 | 42 | 11.6 | 9.3 | 22768 | 5.98 | 2.11 | D | 25 - |
| Ubiquitin carboxyl-terminal hydrolase 1 |
P09936 | 15 | 10 | 11.6 | 17.5 | 24808 | 5.33 | 4.54 | U | – |
| Superoxide dismutase | P00441 | 16 | 15 | 15794 | 5.7 | 0.92 | N | –26 | ||
| Galectin 1 | P09382 | 19 | 20 | 11.6 | 72.4 | 14575 | 5.34 | 0.89 | N | –26 |
| Tumour necrosis factor b prescursor |
P01375 | 20 | 40 | 11.6 | 24.9 | 25628 | 6.88 | 5.91 | D | – |
| Nucleophosmin | P06748 | 26 | 30 | 32555 | 4.64 | 1.67 | N | –26 | ||
| 14 kDa phosphohistidine phosphatase |
Q9NRX4 | 29 | 37 | 13824 | 5.65 | 1.01 | N | – | ||
| Myosin light polypeptide 6 | P60660 | 30 | 33 | 16788 | 4.56 | 1.13 | N | – | ||
| Myosin light polypeptide 6 | P60660 | 31 | 32 | 16788 | 4.56 | 1.29 | N | – | ||
| Aminoacylase-1 | Q03154 | 35 | 22 | 45856 | 5.77 | 0.74 | N | – | ||
| Thioredoxin dependent peroxide |
P30048 | 36 | 16 | 9.9 | 14.1 | 27675 | 8.09 | 1.91 | N | 25 26 |
| Calreticulin precursor, CRP55 f |
P27797 | 1 | 1 | 11.6 | 3.0 | 48112 | 4.29 | 1.28 | N | 25 - |
| Glutathione peroxidase f | P07203 | 18 | 17 | 21885 | 6.50 | 1.16 | N | – | ||
| Galectin 1 f | P09382 | 23 | 19 | 11.6 | 72.4 | 14575 | 5.38 | 1.41 | N | –26 |
| Myosin regulatory light chain f |
P19105 | 24 | 18 | 19650 | 4.66 | 1.53 | N | – | ||
| Calmodulin f | P02593 | 25 | 20 | 10.5 | 23.6 | 16695 | 4.08 | 2.94 | U | – |
| Proactivator polypeptide precursor f |
P07602 | 26 | 21 | 11.6 | 5.3 | 58073 | 5.12 | 3.56 | U | – |
Accession numbers can be accessed through NCBI. To access use the link http://www.ncbi.nlm.nih.gov:80/entrez.query.fcgi?db=Protein
Representative 2DPAGE profiles for these spots are shown in Fig. 1
Representative 2DPAGE profiles for these spots are shown in Fig. 2
Spot data was calculated from spots excised from gels focused at both wide range (pH 3–10) & narrow range (pH 4–7) IEF
This alteration is relative to proteins identified by ESI-MS/MS from ARPE-19 cells exposed to an unmodified matrix and an AGE-modified matrix, with values normalized to control gels. U: significantly upregulated; D: significantly downregulated; N: no significant fold change
These proteins are taken from ARPE-19 cells exposed to unmodified MG and AGE-BM matrices for 1 month
All samples are from a homo sapiens source