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Effect of MRM segmentation and number of transitions on the quantification of glycopeptides depicted for m/z 1308.12. Extracted ion chromatograms (EIC) of NSMRM with 1 transition (A), NSMRM with 2 transitions (B), NSMRM with 3 transition (C), SMRM with 1 transition (D), SMRM with 2 transitions (E), and SMRM with 3 transitions (F). The inset represents quantifications data of 3 MRM LC-MS/MS experiments.
